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Label free cell differentiation in whole blood based on multiple frequency AC impedance and light scatter analysis in a micro flow cytometric sensor

22.06.2016

PTB´s working group 8.32 “Flow Cytometry and Microscopy” has developed a microfluidic sensor for label-free flow cytometric cell differentiation by combined multiple AC electrical impedance and light scattering analysis. The measured signals are correlated to cell volume, membrane capacity and optical properties of single cells. A one-dimensional sheath flow focusing was implemented, which allows single particle analysis at kHz count rates. It has been demonstrated that AC impedance measurements at selected frequencies allow label-free discrimination of platelets, erythrocytes, monocytes, granulocytes and lymphocytes in whole blood samples involving dilution only. Immunofluorescence staining was applied to validate the results of the label-free cell analysis. Reliable differentiation and enumeration of cells in whole blood by AC impedance detection has the potential to support medical diagnosis for patients with haemolysis resistant erythrocytes or abnormally sensitive leucocytes, i.e. for patient suffering from anemia or leukemia.

Publication:

Peter Simon, Marcin Frankowski, Nicole Bock, Jörg Neukammer:
Label-free whole blood cell differentiation based on multiple frequency AC impedance and light scatter analysis in a micro flow cytometer.
Lab on a Chip 16 (2016); DOI: 10.1039/C6LC00128A

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