Title: |
Preparation and characterization of an isotopically enriched (57Fe) ferritin standard for species specific isotope dilution mass spectrometry |
| Author(s): |
A. Tchaikovsky, Anna Schoeberl, S. Emin, Sophie Neumayer and Gunda Koellensperger |
| Year: |
2019 |
| Conference name: |
ReMind 2019 Biomolecules in Neurodegenerative Diseases |
| Conference place: |
Physikalisch-Technische Bundesanstalt, Germany |
| Conference date: |
26./27.06.2019 |
| Abstract: |
The recently introduced EC-directive covering all in vitro diagnostic medical devices (Directive 98/79/EC, In vitro diagnostic medical devices, IVD) demands to assure “the traceability of the values assigned to calibrators and control materials [...] through reference measurement procedures and/or available reference materials of higher order”. At the same time, we face the situation that especially in the case of clinically relevant metallo-proteins, established diagnostic assays lack traceable standards. We present the production pipeline and the characterization of an isotopically enriched (57Fe) ferritin standard. Moreover, we will discuss analytical figures of merit of the methodological tool set based on liquid chromatography inductively coupled plasma mass spectrometry enabled by the standard. More specifically, we will discuss enrichment strategies together with size exclusion chromatography in the context of species specific isotope dilution analysis of ferritin in biologically relevant samples in studies on neurodegenerative diseases. |
