Magnetic nanoparticles navigate therapeutic genes through the body
PTB measures the pinpointed transport of therapeutics for cardiovascular diseases
Magnetic nanoparticles can support or even enable gene transfer under clinically relevant experimental conditions. For the transduction of human cells, gene carriers were coupled to magnetic nanoparticles and dragged into the cells by magnetic field gradients. The efficiency of magnetic transduction turned out to be much higher than the nonmagnetic procedure. An additional welcome side effect is the "magnetization" of the cells after the incorporation of nanoparticles. This may enable the targeted transport of the cells to regions of interest.
A closer look at the underlying mechanism of magnetic gene transfer was taken by the quantification of the magnetic material that was delivered to the cells. The required highly sensitive measurements in the range of a few picogramm per cell were made by PTB using magnetorelaxometry. The good correlation between measurement data and gene transfer encourages to use magnetorelaxometry for monitoring the efficiency of gene and cell transfer, possibly even in vivo.
PTB-contact
Dr. Lutz Trahms,
working group 8.21 Biomagnetism,
phone ++49 (30) 3481-7213,
e-mail: lutz.trahms(at)ptb.de
Originals scientific news on the PTB internet page
http://ib.ptb.de/en/org/8/Nachrichten8/2009/grundlagen/gentransfer.html
Scientific publication in PNAS
PNAS 106 (1), S.44-49: Combined targeting of lentiviral vectors and positioning of transduced cells by magnetic nanoparticles
http://www.pnas.org/gca?allch=&gca=pnas%3B106%2F1%2F44#abstr-1